The Villen Laboratory is open to academic and industry collaborations and offers comprehensive expertise in proteomics analysis:

• Protein identification
• Protein quantification using label free, SILAC, or TMT labeling approaches
• Analysis of post-translational modifications
• Analysis of protein turnover
• Analysis of protein stability
• Custom proteomic assay development

Samples

The laboratory has a wide experience in developing  and implementing sample preparation protocols from a variety of sources and applications:

• Global proteome analysis (from model organisms, cells, and tissue samples).
• Global phosphoproteomes and other PTMs
• Protein complexes (from immuno-affinity purifications): to identify protein interactions, elucidate complex composition, and PTMs
• Purified proteins (in solution or SDS-PAGE gel bands)

Infrastructure

The Villen laboratory is equipped with state of the art facilities for sample preparation:

• Automated and semi-automated robotic sample processing
• Offline HPLC fractionation for deep proteome characterization

Our lab has access to latest-generation mass spectrometry instrumentation:

• Thermo Fisher Scientific Q Exactive (QE)
• Thermo Fisher Scientific Exploris 480
• Thermo Fisher Scientific Eclipse

The laboratory is equipped with In house Computational infrastructure for Data Analysis and Bioinformatic interpretation.

Contact Us

Reach out  to discuss your project’s requirements.

jvillen [ at ] uw.edu