Proteomic Services
The Villen Laboratory is open to academic and industry collaborations and offers comprehensive expertise in proteomics analysis:
- Protein identification
- Protein quantification using label free, SILAC, or TMT labeling approaches
- Analysis of post-translational modifications
- Analysis of protein turnover
- Analysis of protein stability
- Custom proteomic assay development
Samples
The laboratory has a wide experience in developing and implementing sample preparation protocols from a variety of sources and applications:
- Global proteome analysis (from model organisms, cells, and tissue samples).
- Global phosphoproteomes and other PTMs
- Protein complexes (from immuno-affinity purifications): to identify protein interactions, elucidate complex composition, and PTMs
- Purified proteins (in solution or SDS-PAGE gel bands)
Infrastructure
The Villen laboratory is equipped with state of the art facilities for sample preparation:
- Automated and semi-automated robotic sample processing
- Offline HPLC fractionation for deep proteome characterization
Our lab has access to latest-generation mass spectrometry instrumentation:
- Thermo Fisher Scientific Q Exactive (QE)
- Thermo Fisher Scientific Exploris 480
- Thermo Fisher Scientific Eclipse
The laboratory is equipped with In house Computational infrastructure for Data Analysis and Bioinformatic interpretation.
Contact Us
Reach out to discuss your project’s requirements.
jvillen [ at ] uw.edu