{"id":1229,"date":"2023-10-10T15:10:23","date_gmt":"2023-10-10T22:10:23","guid":{"rendered":"https:\/\/villenlab.gs.washington.edu\/wordpress\/?page_id=1229"},"modified":"2023-12-01T10:47:12","modified_gmt":"2023-12-01T18:47:12","slug":"proteomic-services","status":"publish","type":"page","link":"https:\/\/villenlab.gs.washington.edu\/wordpress\/index.php\/proteomic-services\/","title":{"rendered":"Proteomic Services"},"content":{"rendered":"\n<div class=\"wp-block-cover alignfull has-custom-content-position is-position-top-left wp-duotone-rgb929292-rgb238237237-1\"><span aria-hidden=\"true\" class=\"wp-block-cover__background has-nv-site-bg-background-color has-background-dim-20 has-background-dim\"><\/span><img loading=\"lazy\" decoding=\"async\" width=\"4032\" height=\"3024\" class=\"wp-block-cover__image-background wp-image-1332\" alt=\"\" src=\"https:\/\/villenlab.gs.washington.edu\/wordpress\/wp-content\/uploads\/2023\/10\/IMG_20201001_224903689-EDIT.jpg\" style=\"object-position:55% 17%\" data-object-fit=\"cover\" data-object-position=\"55% 17%\"\/><div class=\"wp-block-cover__inner-container is-layout-constrained wp-block-cover-is-layout-constrained\">\n<div class=\"aligncenter blockart-section blockart-section-92477f9e aligncenter\"><div class=\"blockart-container\"><div class=\"blockart-section-inner\">\n<div class=\"blockart-column blockart-column-7fffbca0\"><div class=\"blockart-column-inner\">\n<p class=\"has-nv-text-dark-bg-color has-text-color has-background\" style=\"background:linear-gradient(90deg,rgba(242,246,250,0) 0%,rgba(5,5,5,0.43) 0%);font-size:40px\">Proteomic Services<\/p>\n<\/div><\/div>\n<\/div><\/div><\/div>\n<\/div><\/div>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>The Villen Laboratory is open to academic and industry collaborations and offers comprehensive expertise in proteomics analysis:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Protein identification<\/li>\n\n\n\n<li>Protein quantification using label free, SILAC, or TMT labeling approaches<\/li>\n\n\n\n<li>Analysis of post-translational modifications<\/li>\n\n\n\n<li>Analysis of protein turnover<\/li>\n\n\n\n<li>Analysis of protein stability<\/li>\n\n\n\n<li>Custom proteomic assay development<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Samples<\/h3>\n\n\n\n<p>The laboratory has a wide experience in developing&nbsp; and implementing sample preparation protocols from a variety of sources and applications:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Global proteome analysis (from model organisms, cells, and tissue samples). <\/li>\n\n\n\n<li>Global phosphoproteomes and other PTMs<\/li>\n\n\n\n<li>Protein complexes (from immuno-affinity purifications): to identify protein interactions, elucidate complex composition, and PTMs<\/li>\n\n\n\n<li>Purified proteins (in solution or SDS-PAGE gel bands)<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Infrastructure<\/h3>\n\n\n\n<p>The Villen laboratory is equipped with state of the art facilities for sample preparation:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Automated and semi-automated robotic sample processing<\/li>\n\n\n\n<li>Offline HPLC fractionation\u00a0for deep proteome characterization<\/li>\n<\/ul>\n\n\n\n<p>Our lab has access to latest-generation mass spectrometry instrumentation:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Thermo Fisher Scientific Q Exactive (QE)<\/li>\n\n\n\n<li>Thermo Fisher Scientific Exploris 480<\/li>\n\n\n\n<li>Thermo Fisher Scientific Eclipse<\/li>\n<\/ul>\n\n\n\n<p>The laboratory is equipped with In house Computational infrastructure for Data Analysis and Bioinformatic interpretation.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Contact Us<\/h3>\n\n\n\n<p>Reach out&nbsp; to discuss your project&#8217;s requirements.<\/p>\n\n\n\n<p>jvillen [ at ] uw.edu<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The Villen Laboratory is open to academic and industry collaborations and offers comprehensive expertise in proteomics analysis: Samples The laboratory has a wide experience in developing&nbsp; and implementing sample preparation protocols from a variety of sources and applications: Infrastructure The Villen laboratory is equipped with state of the art facilities for sample preparation: Our lab&hellip;&nbsp;<a href=\"https:\/\/villenlab.gs.washington.edu\/wordpress\/index.php\/proteomic-services\/\" rel=\"bookmark\">Read More &raquo;<span class=\"screen-reader-text\">Proteomic Services<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"neve_meta_sidebar":"","neve_meta_container":"","neve_meta_enable_content_width":"","neve_meta_content_width":0,"neve_meta_title_alignment":"","neve_meta_author_avatar":"","neve_post_elements_order":"","neve_meta_disable_header":"","neve_meta_disable_footer":"","neve_meta_disable_title":"on","footnotes":""},"class_list":["post-1229","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/villenlab.gs.washington.edu\/wordpress\/index.php\/wp-json\/wp\/v2\/pages\/1229","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/villenlab.gs.washington.edu\/wordpress\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/villenlab.gs.washington.edu\/wordpress\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/villenlab.gs.washington.edu\/wordpress\/index.php\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/villenlab.gs.washington.edu\/wordpress\/index.php\/wp-json\/wp\/v2\/comments?post=1229"}],"version-history":[{"count":7,"href":"https:\/\/villenlab.gs.washington.edu\/wordpress\/index.php\/wp-json\/wp\/v2\/pages\/1229\/revisions"}],"predecessor-version":[{"id":1601,"href":"https:\/\/villenlab.gs.washington.edu\/wordpress\/index.php\/wp-json\/wp\/v2\/pages\/1229\/revisions\/1601"}],"wp:attachment":[{"href":"https:\/\/villenlab.gs.washington.edu\/wordpress\/index.php\/wp-json\/wp\/v2\/media?parent=1229"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}